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Monitoring of antiandrogen therapy: Aim is usually to suppress testosterone levels to castrate levels or below (no more than 25% of the lower reference range value).

Females: Decreased testosterone levels may be observed in primary or secondary ovarian failure, analogous to the situation in men, alongside the more prominent changes in female hormone levels.

Most circulating testosterone is bound to sex hormone-binding globulin (SHBG), which in men also is called testosterone-binding globulin.

A lesser fraction is albumin bound and a small proportion exists as free hormone.

Additional measurements of free testosterone or bioavailable testosterone are recommended in this situation; bioavailable testosterone (see TTBS / Testosterone, Total and Bioavailable, Serum) is the preferred assay.